Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations

Summary, in English

We present a xylosylated naphthoxyloside carrying a terminal azide functionality that can be used for conjugation using click chemistry. We show that this naphthoxyloside serves as a substrate for β4GalT7 and induces the formation of soluble glycosaminoglycan (GAG) chains with physiologically relevant lengths and sulfation patterns. Finally, we demonstrate its usefulness by conjugation to the Alexa Fluor 647 and TAMRA fluorophores and coupling to a surface plasmon resonance chip for interaction studies with the hepatocyte growth factor known to interact with the GAG heparan sulfate.

• Författare: Daniel Willén; Roberto Mastio; Zackarias Söderlund; Sophie Manner; Gunilla Westergren-Thorsson; Emil Tykesson; Ulf Ellervik
• Avdelning/ar: Centrum för analys och syntes; Lungbiologi; Lund University Bioimaging Center
• Publiceringsår: 2021
• Språk: Engelska
• Sidor: 2507-2515
• Publikation/Tidskrift/Serie: Bioconjugate Chemistry
• Volym: 32
• Issue: 12
• Länkar: http://dx.doi.org/10.1021/acs.bioconjchem.1c00473
• Dokumenttyp: Artikel i tidskrift
• Förlag: The American Chemical Society (ACS)
• Ämne: Biochemistry and Molecular Biology; Medical Biotechnology
• Status: Published
• Projekt: Small molecules that interfere with the biosynthesis of dermatan sulfate for exploration of cell surface carbohydrates and use as cancer therapy
• Forskningsgrupp: Lung Biology
• ISBN/ISSN/Övrigt: ISSN: 1043-1802