Sophie Manner
Director of studies
Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations
Author
Summary, in English
We present a xylosylated naphthoxyloside carrying a terminal azide functionality that can be used for conjugation using click chemistry. We show that this naphthoxyloside serves as a substrate for β4GalT7 and induces the formation of soluble glycosaminoglycan (GAG) chains with physiologically relevant lengths and sulfation patterns. Finally, we demonstrate its usefulness by conjugation to the Alexa Fluor 647 and TAMRA fluorophores and coupling to a surface plasmon resonance chip for interaction studies with the hepatocyte growth factor known to interact with the GAG heparan sulfate.
Department/s
- Centre for Analysis and Synthesis
- Lung Biology
- Lund University Bioimaging Center
Publishing year
2021
Language
English
Pages
2507-2515
Publication/Series
Bioconjugate Chemistry
Volume
32
Issue
12
Links
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Biochemistry and Molecular Biology
- Medical Biotechnology
Status
Published
Project
- Small molecules that interfere with the biosynthesis of dermatan sulfate for exploration of cell surface carbohydrates and use as cancer therapy
Research group
- Lung Biology
ISBN/ISSN/Other
- ISSN: 1043-1802