The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Sophie Manner

Director of studies

Default user image.

Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations

Author

  • Daniel Willén
  • Roberto Mastio
  • Zackarias Söderlund
  • Sophie Manner
  • Gunilla Westergren-Thorsson
  • Emil Tykesson
  • Ulf Ellervik

Summary, in English

We present a xylosylated naphthoxyloside carrying a terminal azide functionality that can be used for conjugation using click chemistry. We show that this naphthoxyloside serves as a substrate for β4GalT7 and induces the formation of soluble glycosaminoglycan (GAG) chains with physiologically relevant lengths and sulfation patterns. Finally, we demonstrate its usefulness by conjugation to the Alexa Fluor 647 and TAMRA fluorophores and coupling to a surface plasmon resonance chip for interaction studies with the hepatocyte growth factor known to interact with the GAG heparan sulfate.

Department/s

  • Centre for Analysis and Synthesis
  • Lung Biology
  • Lund University Bioimaging Center

Publishing year

2021

Language

English

Pages

2507-2515

Publication/Series

Bioconjugate Chemistry

Volume

32

Issue

12

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Biochemistry and Molecular Biology
  • Medical Biotechnology

Status

Published

Project

  • Small molecules that interfere with the biosynthesis of dermatan sulfate for exploration of cell surface carbohydrates and use as cancer therapy

Research group

  • Lung Biology

ISBN/ISSN/Other

  • ISSN: 1043-1802