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Sophie Manner

Director of studies

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Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans

Author

  • Roberto Mastio
  • Daniel Frankel Willén
  • Zackarias Söderlund
  • Gunilla Westergren-Thorsson
  • Sophie Manner
  • Emil Tykesson
  • Ulf Ellervik

Summary, in English

Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions.

Department/s

  • Centre for Analysis and Synthesis
  • Department Office of Experimental Medical Science

Publishing year

2021-11-23

Language

English

Pages

38283-38292

Publication/Series

RSC Advances

Volume

11

Issue

60

Document type

Journal article

Publisher

Royal Society of Chemistry

Topic

  • Chemical Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 2046-2069