Sophie Manner
Director of studies
Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans
Author
Summary, in English
Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions.
Department/s
- Centre for Analysis and Synthesis
- Department Office of Experimental Medical Science
Publishing year
2021-11-23
Language
English
Pages
38283-38292
Publication/Series
RSC Advances
Volume
11
Issue
60
Document type
Journal article
Publisher
Royal Society of Chemistry
Topic
- Chemical Sciences
Status
Published
ISBN/ISSN/Other
- ISSN: 2046-2069