The course aims to provide advanced theoretical insight into commonly occurring modern separation techniques, such as chromatography, mass spectrometry and capillary electrophoresis. The course also aims to develop the students’ ability to independently select and optimise appropriate separation techniques/methods and to provide a coherent overview of the subject.
On completion of the course the student will be able to:
• provide advanced explanations for the chromatographic principles and techniques that the course includes.
• explain the basics of mass spectrometry and capillary electrophoresis.
• describe the construction and function of different analytical instruments for the techniques treated on the course.
• modify and apply methods and techniques for chromatographic separation, for both quantitative and qualitative purposes.
• evaluate the choice of chromatographic technology for the separation of low molecular weight substances, based on the characteristics of the substances and the sample.
• compile results and present them orally and in writing.
• search, evaluate the relevance of, summarize and present scientific information in an assigned area.
Lectures: Advanced theoretical treatment of chromatographic separation and the underlying distribution and adsorption equilibria. Instrumentation and experimental technology for modern liquid chromatography and gas chromatography. Coupling gas chromatography and liquid chromatography to mass spectrometry. Basic orientation on capillary electrophoresis.
Laboratory work: Experimental technology for high resolution liquid chromatography and mass spectrometry. Optimisation of HPLC systems with different combinations of mobile and stationary phases. Gas chromatography with capillary columns, injection techniques and the use of mass spectrometric detection.
Literature project: Chiral separations with oral and written presentation.