Department of Chemistry

Lund University

Protein mass spectrometry (NAKE 007) 3.0 ECTS

Course description

On completion of the course, participants shall be able to:

Knowledge and understanding

  • Describe the principles of mass spectrometry, including the basic design of the instrument and its operation.
  • Describe the most common mass spectrometry applications, including possibilities and limitations for analysis.
  • Plan and execute the best suited sample preparations and mass spectrometry acquisition.

Skills and abilities

  • Make an experimental plan for preparing and analyzing protein and peptide samples using mass spectrometry.
  • Set up and perform experimental work including purification, concentration and separation of samples.
  • Carry out a mass spectrometry analysis and handle the aquired data using common mass spectrometry software.

Judgement and approach

  • Plan for optimal sample preparations and analysis depending on sample type and aim of the analysis.  
  • Explain which types of results that can be obtained with mass spectrometry.
  • Compare results from different analyses and from an analytical point of view explain differences and similarities.

Course content

The course gives an introduction in how to use protein mass spectrometry as a tool in protein science. Practical parts including protein and peptide sample preparations, deposition with different techniques and the use of chromatographic separations for complex samples prior to mass spectrometry analysis. Manual and automated mass spectrometry acquisition of intact protein and peptide mass determination and determination of peptide sequence information. Basic data handling of mass spectrometry data for protein identification, assessment of PTMs and determination of amino acid sequences.

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